Breakthrough Discovered in Medical Marijuana Cancer Treatment
Researchers learned that cannabinoids have been associated with anti-carcinogenic effects, which are responsible in preventing or delaying the development of cancer.
A new study reveals that Medical Marijuana can be an effective treatment for cancer, that is the word announced by doctors in Germany who concluded that this clarification of the mechanism of cannabinoid action may help investigators to further explore their therapeutic benefit.
The medical article was originally published in the Journal of the National Cancer Institute Advance Access and online on December 25th 2007.
Cancer cells that were treated with combinations of cannabinoids, antagonists of cannabinoid receptors, and small interfering ribo nucleic acid or 'siRNA' to tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) were assessed for invasiveness, protein expression, and activation of signal transduction pathways.
The biggest contribution of this breakthrough discovery, is that the expression of TIMP-1 was shown to be stimulated by cannabinoid receptor activation and to mediate the anti-invasive effect of cannabinoids.
In other words, they learned that treatment with cannabinoids, one of the active ingredients of the medicinal side of marijuana, has been shown to reduce the invasiveness of cancer cells. Prior to now the cellular mechanisms underlying this effect were unclear and the relevance of the findings to the behavior of tumor cells in vivo remains to be determined.
It is already known that marijuana can stimulate the appetite of patients, but researchers have learned that cannabinoids, in addition to having palliative benefits in cancer therapy, have been associated with anti-carcinogenic effects, which are responsible in preventing or delaying the development of cancer.
"Although the anti-proliferative activities of cannabinoids have been intensively investigated, little is known about their effects on tumor invasion," the article stated.
In this now completed round of research, Matrigel-coated and uncoated Boyden chambers were used to quantify invasiveness and migration, respectively, of human cervical cancer 'HeLa' cells that had been treated with cannabinoids.
The stable anandamide analog R(+)-methanandamide 'MA' and the phytocannabinoid 9-tetrahydrocannabinol 'THC' in the presence or absence of antagonists of the CB1 or CB2 cannabinoid receptors or of transient receptor potential vanilloid 1 (TRPV1) or inhibitors of p38 or p42/44 mitogen–activated protein kinase (MAPK) pathways.
A method known as 'reverse transcriptase–polymerase chain reaction' and immunoblotting were used to assess the influence of cannabinoids on the expression of matrix metalloproteinases and endogenous tissue inhibitors. The role of TIMP-1 in the anti-invasive action of cannabinoids was analyzed by transfecting HeLa, human cervical carcinoma, or human lung carcinoma cells cells with siRNA targeting TIMP-1.
They say all statistical tests were two-sided.
Without modifying migration, MA and THC caused a time and concentration-dependent suppression of HeLa cell invasion through Matrigel that was accompanied by increased expression of TIMP-1.
At the lowest concentrations tested, MA and THC led to a decrease in cell invasion.
"The stimulation of TIMP-1 expression and suppression of cell invasion were reversed by pretreatment of cells with antagonists to CB1 or CB2 receptors, with inhibitors of MAPKs, or, in the case of MA, with an antagonist to TRPV1. Knockdown of cannabinoid-induced TIMP-1 expression by siRNA led to a reversal of the cannabinoid-elicited decrease in tumor cell invasiveness in HeLa, A549, and C33A cells."
The researchers concluded that increased expression of TIMP-1 mediates an anti-invasive effect of cannabinoids. That means that in our future, cannabinoids may offer a therapeutic option in the treatment of highly invasive cancers.
Special thanks to the JNCI Journal of the National Cancer Institute, and to Burkhard Hinz, PhD, Institute of Toxicology and Pharmacology, University of Rostock and the affiliation of authors: Institute of Toxicology and Pharmacology, University of Rostock in Rostock, Germany.
The original report published by Oxford University Press was titled, "Inhibition of Cancer Cell Invasion by Cannabinoids via Increased Expression of Tissue Inhibitor of Matrix Metalloproteinases-1Robert Ramer, Burkhard Hinz."
There’s still a lot of confusion across the nation about whether or not marijuana is effective for cancer patients. Odds are you’ve heard something about it but weren’t sure whether the information was reliable or definitive. So, in order to help clear things up, here is a list of 34 studies showing that marijuana cures cancer, categorized by the type of cancers being cured in each study. As you sort through the articles, note that the consistent theme between them is that cannabis shrinks tumors and selectively targets cancer cells. As bills and voter initiatives to legalize medical marijuana spread from state to state, remember that we’re not just talking about mitigating the side effects of chemo (though this is another viable use), we’re talking about curing the cancer itself as well as preventing its spread. I’ve taken the liberty of only including articles from credible scientific journals, removing any biased or otherwise improperly cited studies. Enjoy!
Cures Brain Cancer
http://www.nature.com/bjc/journal/v95/n2/abs/6603236a.html http://www.ncbi.nlm.nih.gov/pubmed/11479216 http://www.jneurosci.org/content/21/17/6475.abstract http://jpet.aspetjournals.org/content/308/3/838.abstract http://mct.aacrjournals.org/content/10/1/90.abstract
Cures Mouth and Throat Cancer
Cures Breast Cancer
http://www.ncbi.nlm.nih.gov/pubmed/20859676 http://www.ncbi.nlm.nih.gov/pubmed/18025276 http://www.ncbi.nlm.nih.gov/pubmed/21915267 http://jpet.aspetjournals.org/content/early/2006/05/25/jpet.106.105247.full.pdf+html http://www.molecular-cancer.com/content/9/1/196 http://www.ncbi.nlm.nih.gov/pubmed/22776349 http://www.pnas.org/content/95/14/8375.full.pdf+html
Cures Lung Cancer
http://www.ncbi.nlm.nih.gov/pubmed/22198381?dopt=Abstract http://www.ncbi.nlm.nih.gov/pubmed/21097714?dopt=Abstract http://www.nature.com/onc/journal/v27/n3/abs/1210641a.html
Cures Uterine, Testicular, and Pancreatic Cancers
Cures Prostate Cancer
http://www.ncbi.nlm.nih.gov/pubmed/12746841?dopt=Abstract http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339795/?tool=pubmed http://www.ncbi.nlm.nih.gov/pubmed/22594963
Cures Colorectal Cancer
Cures Ovarian Cancer
Cures Blood Cancer
http://www.ncbi.nlm.nih.gov/pubmed/12091357 http://www.ncbi.nlm.nih.gov/pubmed/16908594 http://onlinelibrary.wiley.com/doi/10.1002/ijc.23584/abstract http://molpharm.aspetjournals.org/content/70/5/1612.abstract
Cures Skin Cancer
Cures Liver Cancer
Cures Biliary Tract Cancer
Cures Bladder Cancer
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Cures Cancer in General
http://www.ncbi.nlm.nih.gov/pubmed/12514108 http://www.ncbi.nlm.nih.gov/pubmed/15313899 http://www.ncbi.nlm.nih.gov/pubmed/15313899
About the Author
Michael Taillard, professional economist, published author and board member of NORML Nebraska.
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[Last edited Aug 09, 2013 19:30:51]